Downstream Processing in Biotechnology; Selection of unit operation with due consideration of physical, chemical and biochemical aspect of biomolecules; Basic review of downstream process designing;Cell disruption methods: Mechanical & enzymatic methods;Insoluble removal: Filtration, centrifugation; Extraction: Solvent, aqueous two-phase, supercritical; Precipitation; Crystallization; Membrane separations: Ultra filtration, microfiltration, electro dialysis, Reverse osmosis, pervaporation; Chromatographic separations processes; electrophoretic separations;Quality control and quality assurance in biopharmaceutical industry; Good manufacturing practices.